An optimized MLVA assay and its complementarity with PFGE and 1 MLST for Listeria monocytogenes clone identification and 2 surveillance 3 4
نویسندگان
چکیده
8 1 Institut Pasteur, National Reference Centre and World Health Organisation 9 Collaborating Centre for Listeria 10 2 Institut Pasteur, Biology of Infection Unit, Paris, France 11 3 Inserm U1117, Paris, France 12 4 Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France 13 5 Univ Paris-Sud, Institut de Génétique et Microbiologie, UMR 8621, Orsay, France 14 6 CNRS, Orsay, France 15 7 Paris Descartes University, Sorbonne Paris Cité, Institut Imagine, Paris, France 16 8 Necker-Enfants Malades University Hospital, APHP, Division of Infectious Diseases 17 and Tropical Medicine, Paris, France 18 9 Institut Pasteur, Microbial Evolutionary Genomics, Paris, France 19 * Corresponding authors 20 S. Brisse. Microbial Evolutionary Genomics Unit, Institut Pasteur, 28 rue du Dr Roux, 21 F-75724 Paris, France. E-mail: [email protected]; Phone +33 1 40 61 36 58 22 M. Lecuit. Biology of Infection Unit, Institut Pasteur, 25 rue du Dr Roux, F-75724 23 Paris, France. E-mail: [email protected]; Phone +33 1 40 61 30 29 24 25 Copyright © 2013, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.00606-13 JCM Accepts, published online ahead of print on 10 April 2013
منابع مشابه
Optimized Multilocus variable-number tandem-repeat analysis assay and its complementarity with pulsed-field gel electrophoresis and multilocus sequence typing for Listeria monocytogenes clone identification and surveillance.
Populations of the food-borne pathogen Listeria monocytogenes are genetically structured into a small number of major clonal groups, some of which have been implicated in multiple outbreaks. The goal of this study was to develop and evaluate an optimized multilocus variable number of tandem repeat (VNTR) analysis (MLVA) subtyping scheme for strain discrimination and clonal group identification....
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